15180 Mc4r Status: Cancelled
| Gene | Mc4r |
| Chromosome | 18 |
| Deletion Start | 66,860,037 |
| Deletion End | 66,858,967 |
| Deletion Size | 1,071 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,859,861 | 66,859,882 | TCTGGGTGTCATAAGCCTGTTG |
| TUP | 66,859,830 | 66,859,859 | AGAACATTCTAGTGATCGTGGCGATAGCCA |
| TUR | 66,859,806 | 66,859,825 | TGGGTGAGTGCAGGTTCTTG |
| TDF | 66,859,222 | 66,859,244 | TGTTCTACATCTCTTGCCCTCAG |
| TDP | 66,859,193 | 66,859,219 | TCCATACTGCGTGTGCTTCATGTCTCA |
| TDR | 66,859,146 | 66,859,165 | GGTCGATGACGGCGTTACAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTCTGGCTCACAAAGATG |
| SD | TGAGAACAGCTGAGAACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
