15183 Prkaa2 Status: ES cell colonies screened / QC no positives
| Gene | Prkaa2 |
| Chromosome | 4 |
| Deletion Start | 105,109,795 |
| Deletion End | 105,075,418 |
| Deletion Size | 34,378 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,109,206 | 105,109,224 | TGGCCTTGCTGATGAGGTC |
| TUP | 105,109,178 | 105,109,203 | TGCCTTTCCCAGATTCTTCCCTGTCC |
| TUR | 105,109,150 | 105,109,172 | CCTGGTCAACTCCACCATCAAAG |
| TDF | 105,075,758 | 105,075,777 | TGGCTGAGGATGCTGAGATG |
| TDP | 105,075,734 | 105,075,756 | TGGAGGCACAGCTTCTGTGTGGA |
| TDR | 105,075,680 | 105,075,702 | GGCCTGAAGGTCTCCTCTTTTAC |
PCR Primers
| Primer | Sequence |
| SU | CCATCTTCACCGCCCTGG |
| SD | GTAAGACTAGCAAATGCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
