15186 Reg1 Status: ES cell colonies screened / QC no positives
| Gene | Reg1 |
| Chromosome | 6 |
| Deletion Start | 78,428,525 |
| Deletion End | 78,426,334 |
| Deletion Size | 2,192 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,426,912 | 78,426,932 | GGCCAGGAAGCTGAAGAAGAC |
| TUP | 78,426,933 | 78,426,957 | CTGCCATCTGCCAGGATCAGTTGCC |
| TUR | 78,426,964 | 78,426,984 | AGGAGCTGTAGGCATTGGAAC |
| TDF | 78,428,132 | 78,428,152 | AGCAGTGGGTCTCTGTTTCTC |
| TDP | 78,428,155 | 78,428,178 | CAAATCCTGGGCAACTGGGTCTCC |
| TDR | 78,428,184 | 78,428,204 | CACAGTAGCCACGATTGGAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGACACCTTGGTCTCAGCC |
| SD | AGACAAACCAGGCATTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
