15194 Npsr1 Status: ES cell colonies screened / QC no positives
| Gene | Npsr1 |
| Chromosome | 9 |
| Deletion Start | 24,134,862 |
| Deletion End | 24,098,203 |
| Deletion Size | 36,660 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,098,211 | 24,098,230 | CCTCACAGAGGGCAGCTTTC |
| TUP | 24,098,231 | 24,098,254 | ATGCCAACCAGACTGTGCCGATGC |
| TUR | 24,098,297 | 24,098,314 | CAGCCACCAGTGCTTCAG |
| TDF | 24,134,667 | 24,134,687 | ACCACCTGTCTTAGGAAGCAC |
| TDP | 24,134,690 | 24,134,716 | TGGTCCTTTACCTGCTCTGTTTCAAGC |
| TDR | 24,134,783 | 24,134,805 | GGAGAACAGCACAACAGAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | TCCCAATTGCTCCTTAGTGG |
| SD | CTTTAGCATGCTAGGAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
