15212 Gpr65 Status: ES cell colonies screened / QC no positives
| Gene | Gpr65 |
| Chromosome | 12 |
| Deletion Start | 98,276,108 |
| Deletion End | 98,275,093 |
| Deletion Size | 1,016 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,275,396 | 98,275,415 | AGCACGGCGTTCCTCACTTG |
| TUP | 98,275,419 | 98,275,443 | TGCCCTGGACCGCTATTTAGCAGTC |
| TUR | 98,275,481 | 98,275,503 | GAGGCTGGTAATAAACGCGAATC |
| TDF | 98,275,863 | 98,275,886 | GAACGTCAATGACAAGTCTGGATG |
| TDP | 98,275,894 | 98,275,921 | TTTACGGTGTACAGAGTCACAGTAGCCC |
| TDR | 98,275,930 | 98,275,950 | GGGATCGGCAACACAGTTTAG |
PCR Primers
| Primer | Sequence |
| SU | AACAGAGCATTCCCATTTGC |
| SD | GGAGTGGCTTCAAATCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
