15217 Slc26a4 Status: Cancelled
| Gene | Slc26a4 |
| Chromosome | 12 |
| Deletion Start | 31,559,320 |
| Deletion End | 31,529,463 |
| Deletion Size | 29,858 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,559,035 | 31,559,056 | GACAGAGGTGGCTCCAGTAATC |
| TUP | 31,558,999 | 31,559,028 | TGGAATGACTGAAGCTTTGCCTAGAGAAAC |
| TUR | 31,558,971 | 31,558,990 | CGTGACCGGACTCTCACTAC |
| TDF | 31,529,963 | 31,529,984 | TGACTTGAAGCCTCTGATCTAC |
| TDP | 31,529,936 | 31,529,959 | TCTTCCCTCCTCATCGCTTGTGGG |
| TDR | 31,529,872 | 31,529,893 | CCCGGCCTGAAAAGAAATTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTCCTTAAATGGGACTGCC |
| SD | ACAGTTGCAGTTTCCTATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
