Gene | Carf |
Chromosome | 1 |
Deletion Start | 60,132,029 |
Deletion End | 60,108,129 |
Deletion Size | 23,901 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 60,108,766 | 60,108,786 | AAGGGTCACGGCATTAGGAAG |
TUP | 60,108,788 | 60,108,811 | TGGGAACCACTGGCTGAAGACAAC |
TUR | 60,108,821 | 60,108,845 | CAACCATTTACACTGAGCTACATTC |
TDF | 60,131,910 | 60,131,929 | CACAAGCAGCAAACACAGAC |
TDP | 60,131,939 | 60,131,960 | TACTCGCCAGTCTCCAAGCCCA |
TDR | 60,131,981 | 60,132,004 | GGGACATACTGAGACTTCCACATC |
PCR Primers
Primer | Sequence |
SU | GACAGAATGAGAGAACTACG |
SD | GTGATATTGTCTATAAGTTCAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 80 bp | + | + | - |
TDF | TDR | 95 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
15239A-C1 | VGB6 | C57BL/6NTac | |
15239A-G6 | VGB6 | C57BL/6NTac | |