15240 Bard1 Status: ES cell colonies screened / QC no positives
| Gene | Bard1 |
| Chromosome | 1 |
| Deletion Start | 71,102,804 |
| Deletion End | 71,074,548 |
| Deletion Size | 28,257 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 71,102,315 | 71,102,333 | AGGCTAGGGACCTGGTGAC |
| TUP | 71,102,283 | 71,102,311 | TAGTTTGGCGATGGCAGTTTGTTGAGCAT |
| TUR | 71,102,242 | 71,102,263 | TCAGTGGTACAGGGCCATAATC |
| TDF | 71,074,898 | 71,074,919 | TTCGTTCAGGAGGGAGTAATGG |
| TDP | 71,074,870 | 71,074,893 | CGTAAAGGCTGCCACAGGCCTACC |
| TDR | 71,074,824 | 71,074,843 | TGCTGGTGCTCGGAATGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCCTAGGTGACTTCTCCC |
| SD | CTGGACGTGAAGAGGAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
