15258 Rassf5 Status: Cancelled
| Gene | Rassf5 |
| Chromosome | 1 |
| Deletion Start | 131,245,127 |
| Deletion End | 131,212,263 |
| Deletion Size | 32,865 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,244,215 | 131,244,234 | GAATGGAAGCAGGCTGTGAG |
| TUP | 131,244,188 | 131,244,212 | TGAAAGATGCCAAGGTCACCTGCAC |
| TUR | 131,244,136 | 131,244,158 | GGAGCAGAGTATAGAGCGTTGAG |
| TDF | 131,212,560 | 131,212,579 | GCACGGGTGTGAAGCATTTG |
| TDP | 131,212,537 | 131,212,559 | CTGCCACCATTTGTGCCAGTGCA |
| TDR | 131,212,509 | 131,212,531 | GGACTGTGTAAGGGTGACATCTG |
PCR Primers
| Primer | Sequence |
| SU | AGTCACTCCTAAATCCACGC |
| SD | TGGAGCTTCAGGTCTTCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
