| Gene | Batf3 |
| Chromosome | 1 |
| Deletion Start | 191,108,695 |
| Deletion End | 191,098,462 |
| Deletion Size | 10,234 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 191,099,272 | 191,099,291 | TCTGTGGCAGGTGATGTGAC |
| TUP | 191,099,294 | 191,099,316 | CTCCCAGAGGACAGGAGGCATGC |
| TUR | 191,099,334 | 191,099,358 | GTCTCTATTCTCCAAAGCCAACAAG |
| TDF | 191,108,008 | 191,108,029 | GACTGCATGTGTCTGTGCATTG |
| TDP | 191,108,034 | 191,108,056 | TACAGCCGAAGGCATGTCGTCCT |
| TDR | 191,108,064 | 191,108,084 | AGCCATCCCAACACCAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACGTGACTTCCACGAAGAGC |
| SD | TCCCACGGTCTTAACTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15272A-E3 | VGB6 | C57BL/6NTac | |
| 15272A-F11 | VGB6 | C57BL/6NTac | |
| 15272A-G5 | VGB6 | C57BL/6NTac | |
