15273 Cr1l Status: ES cell colonies screened / QC no positives
| Gene | Cr1l |
| Chromosome | 1 |
| Deletion Start | 195,131,302 |
| Deletion End | 195,103,856 |
| Deletion Size | 27,447 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 195,130,610 | 195,130,632 | CATTAACTCCCTTGAGCTTCCAC |
| TUP | 195,130,581 | 195,130,606 | AAGGAAGGTCCTTCCACTCCCACTCC |
| TUR | 195,130,538 | 195,130,558 | TTCATTGCACCTTGAGGCTTG |
| TDF | 195,104,702 | 195,104,724 | TCCCTATCCATAACCCTGAGTTC |
| TDP | 195,104,673 | 195,104,696 | TGCCATATTCCACTTGGGCAGCTC |
| TDR | 195,104,642 | 195,104,666 | GAAACATGAAAACATAGCCAGTTGG |
PCR Primers
| Primer | Sequence |
| SU | CAGGGTATGCAACTGTCAGC |
| SD | AAGGACTTGTGCTTAGTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
