15284 Scn7a Status: ES cell colonies screened / QC no positives
| Gene | Scn7a |
| Chromosome | 2 |
| Deletion Start | 66,757,354 |
| Deletion End | 66,729,017 |
| Deletion Size | 28,338 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,756,484 | 66,756,506 | TCTAATAGCACTGGCTGTTCTTC |
| TUP | 66,756,456 | 66,756,483 | TGGACGTCATAAGATTGATTGCAGTCCC |
| TUR | 66,756,428 | 66,756,451 | GTTACAAAAGGCTGTGACCTACTG |
| TDF | 66,729,486 | 66,729,508 | CATGTCACTGGCACTAGGTTCTC |
| TDP | 66,729,455 | 66,729,482 | ACAGAAAGGCTGGCTCAAGTTAGGAAGA |
| TDR | 66,729,416 | 66,729,436 | CCCGATGGTATCCCTGAACTG |
PCR Primers
| Primer | Sequence |
| SU | GCGACATATTCATGGATCAGC |
| SD | TAGGGCTAGTCTCAAGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
