15300 Aqr Status: Cancelled
| Gene | Aqr |
| Chromosome | 2 |
| Deletion Start | 114,175,163 |
| Deletion End | 114,145,632 |
| Deletion Size | 29,532 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,174,552 | 114,174,575 | AGCCTGTTCTTCAGATTGCTCCAG |
| TUP | 114,174,529 | 114,174,551 | AGTGCTCACTGGTGCAAACCTGC |
| TUR | 114,174,436 | 114,174,456 | AGGCCAGATCACAGCACTTTC |
| TDF | 114,146,022 | 114,146,041 | CTGCATGTGGGAGTGAGGAG |
| TDP | 114,145,997 | 114,146,018 | CTACAGGAGCTGCTGGGCCTCG |
| TDR | 114,145,947 | 114,145,969 | GACAGATAGCACGGACCTTCTAC |
PCR Primers
| Primer | Sequence |
| SU | TATGACCCAATCCTACACGC |
| SD | ACACAGAAGCTGCTCAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 140 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
