15301 Atpbd4 Status: ES cell colonies screened / QC no positives
| Gene | Atpbd4 |
| Chromosome | 2 |
| Deletion Start | 114,654,850 |
| Deletion End | 114,644,725 |
| Deletion Size | 10,126 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,654,418 | 114,654,435 | TTGCCGGGAGAGCTGTAG |
| TUP | 114,654,385 | 114,654,413 | CCCTAGATGGTTTGAGATTGCTTTGCTCC |
| TUR | 114,654,356 | 114,654,374 | GAGTTCCAACGGAGGTGTC |
| TDF | 114,644,837 | 114,644,859 | TGACTTGTATGCTGAAGCAATGG |
| TDP | 114,644,806 | 114,644,830 | CCCTGTATCGCAGAGCCATCAGAGG |
| TDR | 114,644,773 | 114,644,796 | TGCGTATAAACTCTTCCTGTCTCC |
PCR Primers
| Primer | Sequence |
| SU | TCAAGCGGTTTACCAAGGAC |
| SD | GTAAAAAGCGTGTGTCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
