15306 Nanp Status: ES cell colonies screened / QC no positives
| Gene | Nanp |
| Chromosome | 2 |
| Deletion Start | 151,039,323 |
| Deletion End | 151,030,237 |
| Deletion Size | 9,087 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,038,399 | 151,038,418 | TCACTGGGACGTAGCTTCTG |
| TUP | 151,038,368 | 151,038,394 | TGCCACAAGAATACAATAGGCTTGCCA |
| TUR | 151,038,343 | 151,038,363 | GCACTTTGGGAGCCACCTAAC |
| TDF | 151,030,984 | 151,031,008 | GAGATTGTGTGGTAAAGGGAAGAAG |
| TDP | 151,030,963 | 151,030,983 | TCGCTCCTTCTTAAGACATGA |
| TDR | 151,030,937 | 151,030,960 | TGGTCAGCATACCTCGTCTTTAAC |
PCR Primers
| Primer | Sequence |
| SU | CCATAGAAAGACAGGCCAGC |
| SD | ACTTATATTGTCCCGATTCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
