| Gene | Arpm1 |
| Chromosome | 3 |
| Deletion Start | 30,599,846 |
| Deletion End | 30,597,828 |
| Deletion Size | 2,019 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,599,458 | 30,599,478 | TCAAGCATACAGTGGCCGTTC |
| TUP | 30,599,428 | 30,599,452 | AGATGTGCCTGCGTAAGAATGTGGC |
| TUR | 30,599,391 | 30,599,411 | CAAGAGACCACACCGCATGAC |
| TDF | 30,598,652 | 30,598,671 | GAACCTGAACGCCAGTGATG |
| TDP | 30,598,625 | 30,598,648 | CGGTCTTGGTTACAGAACCTGCGC |
| TDR | 30,598,605 | 30,598,623 | CCGGTCTGCCAGTGGATTC |
PCR Primers
| Primer | Sequence |
| SU | AAGGGTCTTCCTCCAGCTTC |
| SD | TTTCCTGCCCTGTTCAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15324A-A6 | VGB6 | C57BL/6NTac | |
| 15324A-B10 | VGB6 | C57BL/6NTac | |
| 15324A-C1 | VGB6 | C57BL/6NTac | |
| 15324A-C9 | VGB6 | C57BL/6NTac | |
| 15324A-F3 | VGB6 | C57BL/6NTac | |
| 15324A-H4 | VGB6 | C57BL/6NTac | |
