15327 A730037C10Rik Status: ES cell colonies screened / QC no positives
| Gene | A730037C10Rik |
| Chromosome | 3 |
| Deletion Start | 55,171,461 |
| Deletion End | 55,150,798 |
| Deletion Size | 20,664 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,151,297 | 55,151,318 | ACACATGAATCAGCACAGCTAC |
| TUP | 55,151,320 | 55,151,345 | AGGTCTGCCGGACATTGACACAAAGT |
| TUR | 55,151,371 | 55,151,392 | GGGACACTAACAGGCGACATTG |
| TDF | 55,170,995 | 55,171,013 | GACACGGGCTTCCACATTG |
| TDP | 55,171,015 | 55,171,036 | CTACCCGACGCCTGCTTCTGGA |
| TDR | 55,171,044 | 55,171,064 | GCAAAGGCAGGTACTCAACAG |
PCR Primers
| Primer | Sequence |
| SU | AGGGCAGAAGGAAACATGC |
| SD | TGCATGTGATCTGGGAATATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
