15339 F3 Status: ES cell colonies screened / QC no positives
| Gene | F3 |
| Chromosome | 3 |
| Deletion Start | 121,734,397 |
| Deletion End | 121,723,718 |
| Deletion Size | 10,680 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,723,920 | 121,723,937 | TGTCCGGCTGTACCTGTG |
| TUP | 121,723,939 | 121,723,960 | ATTTGGCTGCGCTCGCCCTGAG |
| TUR | 121,723,966 | 121,723,990 | CTACCTTCCATCCCATTGTTCATTC |
| TDF | 121,734,196 | 121,734,215 | GAGCGGGACAGAAAGGGAAG |
| TDP | 121,734,221 | 121,734,240 | CCCGTCGCGCTTGGCATAGA |
| TDR | 121,734,257 | 121,734,276 | GCAGGCAGTGTGAGCGTTAG |
PCR Primers
| Primer | Sequence |
| SU | CGGAGTTTCCTACAGGAGGG |
| SD | GCCACTTGCTTTGAACTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
