15345 Ints8 Status: Cancelled
| Gene | Ints8 |
| Chromosome | 4 |
| Deletion Start | 11,254,107 |
| Deletion End | 11,225,607 |
| Deletion Size | 28,501 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,253,277 | 11,253,297 | TGGCATTAGCCGCTACTTTAC |
| TUP | 11,253,248 | 11,253,276 | TGGTGGGAAACAAAGCTAGAGACAGTTCA |
| TUR | 11,253,170 | 11,253,192 | GCATTACAGGTTCCCAGTTCAAG |
| TDF | 11,225,745 | 11,225,765 | GGAGCTGGTGACAGAGTTCTC |
| TDP | 11,225,710 | 11,225,735 | CCGCCAGGTTATGCTGAATGAGATGC |
| TDR | 11,225,676 | 11,225,694 | ACTCCAGCCTCGTGAGTGC |
PCR Primers
| Primer | Sequence |
| SU | CAGAATCTAGCTTCTGAAGG |
| SD | AACTAGAAACTAGGAAGTAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
