15360 Raver2 Status: Cancelled
| Gene | Raver2 |
| Chromosome | 4 |
| Deletion Start | 101,150,475 |
| Deletion End | 101,123,277 |
| Deletion Size | 27,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,069,443 | 101,069,462 | AGCCCTTTGCCTCGACGAAG |
| TUP | 101,069,465 | 101,069,485 | TTTCTGCAGCCAGCGAGAGCA |
| TUR | 101,069,495 | 101,069,514 | CCTTGCCCAGCCTCAGTTTC |
| TDF | 101,095,976 | 101,095,994 | GGTGGGATTCCCGTGTGAC |
| TDP | 101,096,004 | 101,096,031 | CCTGGGCAGCTTTGGTGACTAGTTTACC |
| TDR | 101,096,052 | 101,096,072 | CTGCACCTCGCTTTGTACTTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGGCCCTCGGATTTGAG |
| SD | AACTTTAGATGGAAGCATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
