15366 Ctps Status: ES cell colonies screened / QC no positives
| Gene | Ctps |
| Chromosome | 4 |
| Deletion Start | 120,567,412 |
| Deletion End | 120,540,907 |
| Deletion Size | 26,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,566,955 | 120,566,979 | GGAAGATGGAATATGCTGCTTTGTG |
| TUP | 120,566,927 | 120,566,954 | TCCCAATGTGTAGAAATTGCATGGGTGA |
| TUR | 120,566,894 | 120,566,913 | CAAGGCCAAAACCCAGTCTC |
| TDF | 120,541,030 | 120,541,048 | CACAGGCCAGCCAGCTTAG |
| TDP | 120,541,004 | 120,541,028 | TCTCCCTGTGAGCTGCAGATTCTGA |
| TDR | 120,540,942 | 120,540,963 | AAGACAGAGGTGGACAGACATC |
PCR Primers
| Primer | Sequence |
| SU | TGTGAGAGATTGCTGCCAAG |
| SD | AAAGTGTCAGAACTTAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
