15367 Mycbp Status: ES cell colonies screened / QC no positives
| Gene | Mycbp |
| Chromosome | 4 |
| Deletion Start | 123,911,141 |
| Deletion End | 123,905,076 |
| Deletion Size | 6,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,905,520 | 123,905,539 | GCAGTGGACACGTTAGCAAG |
| TUP | 123,905,541 | 123,905,565 | CCTTGAGATCCCAGTTGCTCCCTGC |
| TUR | 123,905,566 | 123,905,589 | TTGTAAAATCCCGCACACTATACG |
| TDF | 123,910,377 | 123,910,399 | GTAGGCCACTGCTTTGTTAAGAC |
| TDP | 123,910,405 | 123,910,429 | CTGCACTGCTAGTTCCAGGTCCTCA |
| TDR | 123,910,434 | 123,910,455 | TCGAGTGCAGCTTACTTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGAGCCTCTAACTCCTGGC |
| SD | AAAAGTTATGTGGCTCTTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
