| Gene | BC046331 |
| Chromosome | 4 |
| Deletion Start | 153,992,988 |
| Deletion End | 153,978,986 |
| Deletion Size | 14,003 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,979,042 | 153,979,063 | TGCCCGAGAACTAATGATCCAC |
| TUP | 153,979,065 | 153,979,087 | CACCAACCGTCAGTGGGTGGAGA |
| TUR | 153,979,092 | 153,979,112 | CCTCAGCCCAGCCTTACTTTG |
| TDF | 153,992,063 | 153,992,082 | GCACCCAGGTGTGACTTCTC |
| TDP | 153,992,085 | 153,992,110 | ATCCACCCTCCTGTGAGTCAGCTCCA |
| TDR | 153,992,116 | 153,992,140 | CACTTGTGCTCTGTGTCTGTGTATG |
PCR Primers
| Primer | Sequence |
| SU | AAGGATGTGATTCAGCTCGC |
| SD | CATGTCCAGTGAGTGCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15375C-H8 | VGB6 | C57BL/6NTac | |
| 15375C-F6 | VGB6 | C57BL/6NTac | |
| 15375B-C11 | VGB6 | C57BL/6NTac | |
| 15375B-D8 | VGB6 | C57BL/6NTac | |
