15378 Actr3b Status: ES cell colonies screened / QC no positives
| Gene | Actr3b |
| Chromosome | 5 |
| Deletion Start | 25,798,497 |
| Deletion End | 25,760,088 |
| Deletion Size | 38,410 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,760,556 | 25,760,577 | TCCCTACTGATGTACCAACCAC |
| TUP | 25,760,580 | 25,760,605 | ACTCGTGGAGATGTTTACACGTGCCA |
| TUR | 25,760,649 | 25,760,669 | AGGCTGTGCCATTTCAGAGTG |
| TDF | 25,797,849 | 25,797,869 | TGGCAAAGAGATGCGCCTATC |
| TDP | 25,797,873 | 25,797,897 | TGATCCTCAGGAGATGGCAGGAGCT |
| TDR | 25,797,968 | 25,797,988 | GGCTCTGTGCAAAGGACTTAC |
PCR Primers
| Primer | Sequence |
| SU | AGAAAGAATTCAGGCCTCGC |
| SD | CGGAGGTGAAACTAGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 140 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
