| Gene | Hgfac |
| Chromosome | 5 |
| Deletion Start | 35,048,404 |
| Deletion End | 35,041,600 |
| Deletion Size | 6,805 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,042,198 | 35,042,217 | GGAGTCTCAGGTCTCTGGTG |
| TUP | 35,042,218 | 35,042,243 | TATCCGACTGGTGGAGTCCAAGTGTG |
| TUR | 35,042,247 | 35,042,270 | CAAAGGTGTCAGTGTGGAATAGTC |
| TDF | 35,047,734 | 35,047,756 | GAACTTGATGCTGGGACAATGAG |
| TDP | 35,047,759 | 35,047,787 | CTTCCCAGGAAAGAGGACAGTAGCACTGG |
| TDR | 35,047,821 | 35,047,842 | CCACCGTATGAAATGCCTCCTC |
PCR Primers
| Primer | Sequence |
| SU | GTCAGTAGCTGGCTGGGAAG |
| SD | ACCTGTGGAACTGGACTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15382A-B7 | VGB6 | C57BL/6NTac | |
| 15382A-C9 | VGB6 | C57BL/6NTac | |
| 15382A-G11 | VGB6 | C57BL/6NTac | |
| 15382A-G7 | VGB6 | C57BL/6NTac | |
| 15382A-F12 | VGB6 | C57BL/6NTac | |
