15393 Sart3 Status: ES cell colonies screened / QC no positives
| Gene | Sart3 |
| Chromosome | 5 |
| Deletion Start | 113,771,632 |
| Deletion End | 113,743,115 |
| Deletion Size | 28,518 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,771,141 | 113,771,161 | GGGCGTCCCTAAAGTATTCCA |
| TUP | 113,771,115 | 113,771,137 | AATAGTGTTTAATCCGGTTTCCC |
| TUR | 113,771,091 | 113,771,111 | CCCTCAACGAGCCTCAGTTTC |
| TDF | 113,743,357 | 113,743,381 | TCTCATAATCCCAACATCTCAGTCC |
| TDP | 113,743,323 | 113,743,348 | CCATGAGACCATGCTGCCAAAGGGTC |
| TDR | 113,743,300 | 113,743,319 | GCGCCTAGAAGAGCAAACAC |
PCR Primers
| Primer | Sequence |
| SU | CAGCTGTCCACGGTTGTAG |
| SD | TCTCCCATTCCATCTTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
