15409   Hoxa10     Status: ES cell colonies screened / QC one positive

GeneHoxa10
Chromosome6
Deletion Start52,234,880
Deletion End52,232,411
Deletion Size2,470
Genome Build38
MGI IDMGI:96171
NCBI Gene ID15395
BAC ID245F13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCCAGCCCCTTCAGAAAACAG
TUPAAAGCTTCGCCGGAGAAGGACTCC
TURGACTTGAGCCCTCGGTTGAG
TDFGCGATCTAGGAGGCTAGGGTAAG
TDPCCAGTGGAGCTGCCAGCCTCTC
TDRGGGTAAGGGAATGCTTCTTTGG

PCR Primers

PrimerSequence
SUACCTCCACTTTGAGGCAG
SDTCCCTGATTAAACACAGCCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15409A-H6VGB6C57BL/6NTac