15432 Zim1 Status: Cancelled
| Gene | Zim1 |
| Chromosome | 7 |
| Deletion Start | 6,688,669 |
| Deletion End | 6,676,911 |
| Deletion Size | 11,759 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,688,217 | 6,688,235 | AGGCCCAGGGATCGAACTC |
| TUP | 6,688,190 | 6,688,213 | TCCTCAGTCCTAGAAGCCGACCGA |
| TUR | 6,688,157 | 6,688,179 | CCAGGCCAACAGGATCTTATCAG |
| TDF | 6,677,683 | 6,677,703 | GGGAGTGAGACCCTACAAGTG |
| TDP | 6,677,657 | 6,677,681 | AAGGAGTGTGGCAAAGCCTTCAACA |
| TDR | 6,677,630 | 6,677,652 | TGCAGTGATTGTTGAGAGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | ATAGGAAATGCCTGTCTCGG |
| SD | ATGTCTCATTAGTGGTTACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
