15448 Zfp84 Status: ES cell colonies screened / QC no positives
| Gene | Zfp84 |
| Chromosome | 7 |
| Deletion Start | 29,777,596 |
| Deletion End | 29,771,374 |
| Deletion Size | 6,223 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,771,388 | 29,771,408 | CAGCACCGTGTTTAAGGTGAG |
| TUP | 29,771,410 | 29,771,437 | TGAATTTCTCCTGCCAGCTCTCTTCTGG |
| TUR | 29,771,441 | 29,771,465 | GGTGCAAGTCCATTTCAGGAATTAG |
| TDF | 29,776,916 | 29,776,938 | GAGTGCCAACCTCACTAGACATC |
| TDP | 29,776,941 | 29,776,964 | AGGATCCACAGCGGCGAGAAACCC |
| TDR | 29,776,970 | 29,776,993 | AAGGTCTTTCCGCATAAGTTACAC |
PCR Primers
| Primer | Sequence |
| SU | CTGAAGGAATGAGGGTCTGC |
| SD | CAGATGGTTCTCTCTGACAAACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
