15454 Gabrg3 Status: Cancelled
| Gene | Gabrg3 |
| Chromosome | 7 |
| Deletion Start | 57,386,770 |
| Deletion End | 57,381,595 |
| Deletion Size | 5,176 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,386,059 | 57,386,079 | GGGCTCAGAAGAAGGACAGTG |
| TUP | 57,386,031 | 57,386,057 | CACCAGAAGGGTTAAAGCCTGTTGCCA |
| TUR | 57,386,004 | 57,386,026 | CCATGTCAGCATCTCTAGTTGTG |
| TDF | 57,382,135 | 57,382,154 | GGCTGCTCCTACTCCAACTG |
| TDP | 57,382,094 | 57,382,123 | TGGCCATGCACTCATAATCCATCTACTCCA |
| TDR | 57,382,068 | 57,382,089 | GAAGAGGAGGTGGCAAGATCAC |
PCR Primers
| Primer | Sequence |
| SU | ACGCTTCGGAGCGCAGCT |
| SD | TAAGACAGGGAACACAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
