15473 Rnh1 Status: Cancelled
| Gene | Rnh1 |
| Chromosome | 7 |
| Deletion Start | 141,166,765 |
| Deletion End | 141,160,434 |
| Deletion Size | 6,332 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,166,742 | 141,166,763 | TCTTGACATCCAGTGTGAGCAG |
| TUP | 141,166,715 | 141,166,736 | TGATGCCCGGTGGACAGAGCTC |
| TUR | 141,166,677 | 141,166,699 | TCCTACCTGACCACTTCGTATTG |
| TDF | 141,161,042 | 141,161,062 | CCTGTCTACCGTGTCACTTTC |
| TDP | 141,161,011 | 141,161,037 | TGCATCCTGATGTAGTTTGGCCTTGGT |
| TDR | 141,160,959 | 141,160,983 | GCATATCCATGTCTCTTAGCACCAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTGGTATCCTGGTGCTGG |
| SD | AGGCCATCTTTGAGACTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
