15491 Phkb Status: ES cell colonies screened / QC no positives
| Gene | Phkb |
| Chromosome | 8 |
| Deletion Start | 85,879,804 |
| Deletion End | 85,843,064 |
| Deletion Size | 36,741 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,843,259 | 85,843,282 | TGCCCATTGAGTTTTGAATGTATC |
| TUP | 85,843,284 | 85,843,307 | TGTGGTACCCCAGGAAGGCAAAGG |
| TUR | 85,843,359 | 85,843,380 | GACAGTTGCAGTTCCTAAGCAC |
| TDF | 85,879,708 | 85,879,729 | CAGGCGCATTGATGATGACAAG |
| TDP | 85,879,732 | 85,879,753 | ACGGACCCATGAGCTGGAGCAC |
| TDR | 85,879,781 | 85,879,800 | TGCCGCATGTAGCAGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTTTCTGGGACAGGCTCCTC |
| SD | CACTTGATGATGTTACGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
