15499 Zfhx3 Status: Cancelled
| Gene | Zfhx3 |
| Chromosome | 8 |
| Deletion Start | 108,800,686 |
| Deletion End | 108,792,251 |
| Deletion Size | 8,436 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,793,120 | 108,793,141 | TGGGTATGTGCGTTCATTTGTG |
| TUP | 108,793,142 | 108,793,162 | ACCCACGCGGTGCACGACCAT |
| TUR | 108,793,164 | 108,793,184 | CCTCTTCGCTCAGGGTCATTC |
| TDF | 108,800,243 | 108,800,262 | TGTCGGAGGAGCTGATGAAC |
| TDP | 108,800,264 | 108,800,286 | TGGGCGAGAGCTTCATCCAGACC |
| TDR | 108,800,307 | 108,800,325 | GCAGACGGCACATTGGAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGTCTCAAAGCCCTGATG |
| SD | GGAGCACAGAGGAGTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
