15505 Casp12 Status: ES cell colonies screened / QC no positives
| Gene | Casp12 |
| Chromosome | 9 |
| Deletion Start | 5,358,854 |
| Deletion End | 5,345,522 |
| Deletion Size | 13,333 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,345,608 | 5,345,630 | GCAGAGCTGTTTGTAAGTTGAGG |
| TUP | 5,345,637 | 5,345,664 | AGGCTATGGTAGCACATGTTGAAGCAGA |
| TUR | 5,345,678 | 5,345,701 | GCTGAGGATAAATGCCTTTGAGAG |
| TDF | 5,358,124 | 5,358,148 | GAGGTCTTTGCTTTACTCTCCTTTG |
| TDP | 5,358,150 | 5,358,178 | CCTCCTTTCTTCTGTGAAGCACCCAATCA |
| TDR | 5,358,185 | 5,358,205 | TGGGCATGACAGTTTAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | CAAGTGTCACGGTCTCATGG |
| SD | AGAGAGGGTTGTACTTTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
