15524 Agphd1 Status: Cancelled
| Gene | Agphd1 |
| Chromosome | 9 |
| Deletion Start | 54,947,105 |
| Deletion End | 54,920,482 |
| Deletion Size | 26,624 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,920,710 | 54,920,733 | CCAGAGCTGATTGAAATGCAGAAC |
| TUP | 54,920,738 | 54,920,763 | TCATCATGTTCCTGAGAGCAGCTGGA |
| TUR | 54,920,768 | 54,920,786 | CGGCATACGGAGGCTGTTG |
| TDF | 54,946,126 | 54,946,146 | CTGGAGAAGGTGTGCATCAAG |
| TDP | 54,946,157 | 54,946,184 | TTTAGACTTCGGTGACATGAGCTATGGC |
| TDR | 54,946,187 | 54,946,211 | AACGATTGCCACTTCAAACACATAG |
PCR Primers
| Primer | Sequence |
| SU | AGGTCATCTGCTTCTTCACCTT |
| SD | GGCAAGGGTTCATCATGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
