| Gene | Bves |
| Chromosome | 10 |
| Deletion Start | 45,369,388 |
| Deletion End | 45,339,234 |
| Deletion Size | 30,155 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,339,669 | 45,339,689 | TGCTCAGGTTCTATGCGTCAG |
| TUP | 45,339,698 | 45,339,727 | TTCATGCGCAAGTATGTAGCAGATGTTGGA |
| TUR | 45,339,730 | 45,339,750 | GGGCTGGGATCACATACTCTG |
| TDF | 45,369,273 | 45,369,292 | GTCTCCAGACGCACTCAAAG |
| TDP | 45,369,293 | 45,369,316 | TCCATCAGTTGCCTTGAGCGGCAT |
| TDR | 45,369,342 | 45,369,363 | GGACTTCTCCTGCTCACTCATC |
PCR Primers
| Primer | Sequence |
| SU | TTACATTGTCGGAAATTGGCG |
| SD | CCCAGTGAGGGAATCACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15554A-A11 | VGB6 | C57BL/6NTac | |
| 15554A-B12 | VGB6 | C57BL/6NTac | |
| 15554A-G4 | VGB6 | C57BL/6NTac | |
