| Gene | Ddx21 |
| Chromosome | 10 |
| Deletion Start | 62,602,211 |
| Deletion End | 62,582,341 |
| Deletion Size | 19,871 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,601,403 | 62,601,421 | CAAACCTTGGGCAGCTCAC |
| TUP | 62,601,380 | 62,601,401 | TGTTGGCAGGTGCTGTCCCACT |
| TUR | 62,601,356 | 62,601,375 | CGCTGGGCTAAGGGCTAGAG |
| TDF | 62,582,786 | 62,582,807 | ACCTCAGACAGATGCAGCAAAC |
| TDP | 62,582,751 | 62,582,777 | TAGAATGAGGACTGGCCTTCAGAAGGC |
| TDR | 62,582,723 | 62,582,743 | CAAAGCCATGTGGCTAGTGTG |
PCR Primers
| Primer | Sequence |
| SU | GGGTTTATCTGTCACCACCC |
| SD | CTCCAGAGACTGAGGCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15557A-B12 | VGB6 | C57BL/6NTac | |
| 15557A-C12 | VGB6 | C57BL/6NTac | |
| 15557A-D9 | VGB6 | C57BL/6NTac | |
| 15557A-E12 | VGB6 | C57BL/6NTac | |
| 15557A-G1 | VGB6 | C57BL/6NTac | |
