15568 Cdc34 Status: Cancelled
| Gene | Cdc34 |
| Chromosome | 10 |
| Deletion Start | 79,688,152 |
| Deletion End | 79,682,417 |
| Deletion Size | 5,736 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,683,223 | 79,683,243 | CACCTGCTCCAGTTCCAGTAG |
| TUP | 79,683,248 | 79,683,270 | CCTGATAGGGCCTTGGCAGCCTT |
| TUR | 79,683,282 | 79,683,303 | CTAGGGCTAAAGGTGGCTGTTG |
| TDF | 79,687,269 | 79,687,289 | ATGTGTTGCTTCACCCGTATC |
| TDP | 79,687,292 | 79,687,316 | TGTCACTTCATCCATGGCCTGTGGG |
| TDR | 79,687,334 | 79,687,354 | GAAGGGCAGTCCCAGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGCACGCAAGCGCTTTCG |
| SD | CTATCTCTTCCTGCCCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
