15569 Hcn2 Status: ES cell colonies screened / QC no positives
| Gene | Hcn2 |
| Chromosome | 10 |
| Deletion Start | 79,735,579 |
| Deletion End | 79,716,672 |
| Deletion Size | 18,908 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,717,463 | 79,717,481 | AGTTGCAGTGGGCGAGAAC |
| TUP | 79,717,487 | 79,717,507 | CCTCAGGGCCAGGGTCTGAGC |
| TUR | 79,717,566 | 79,717,587 | CCCATCTTTCCACCCTCATTTC |
| TDF | 79,734,949 | 79,734,973 | TGTCAAATATGACCGTGAGATGGTG |
| TDP | 79,734,974 | 79,734,993 | CAGCAGGCAGAGCTTGGCCA |
| TDR | 79,735,042 | 79,735,061 | CCTGCTGTAGGGTGGCAATG |
PCR Primers
| Primer | Sequence |
| SU | TCTGCATCGTGCGTCAAGG |
| SD | TCGTCAGTACATTCATGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
