15575 Slc6a15 Status: ES cell colonies screened / QC no positives
| Gene | Slc6a15 |
| Chromosome | 10 |
| Deletion Start | 103,418,448 |
| Deletion End | 103,389,056 |
| Deletion Size | 29,393 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,389,497 | 103,389,517 | GTCCAGCTCTGGGTTCAGATG |
| TUP | 103,389,522 | 103,389,549 | TTCCATAGGAACAGATTGGCCTCCTACA |
| TUR | 103,389,570 | 103,389,593 | GACAAAGTTTGGGAATGTGTTACC |
| TDF | 103,418,322 | 103,418,341 | TGCTCCCAATGGACGCTATG |
| TDP | 103,418,346 | 103,418,370 | TGGGTATTTGATGGCAGACATGCCA |
| TDR | 103,418,388 | 103,418,409 | CCTGCTTTCGTTCAGCTACAAG |
PCR Primers
| Primer | Sequence |
| SU | GAATGACCAAGATCTATCGTG |
| SD | GCTCACACAACTGTTCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
