| Gene | Ascc2 |
| Chromosome | 11 |
| Deletion Start | 4,672,491 |
| Deletion End | 4,640,403 |
| Deletion Size | 32,089 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,640,420 | 4,640,442 | CCAACTCCAGATCACCCACAAAG |
| TUP | 4,640,443 | 4,640,466 | ACCCGAAGACAGGACAGCCGAAGA |
| TUR | 4,640,481 | 4,640,499 | CATCAGGGCATCGGCTCAC |
| TDF | 4,672,234 | 4,672,251 | CCTTGCCCAGCTTAGTCC |
| TDP | 4,672,254 | 4,672,276 | CCCTGCATCACTCTGGCTCTCCA |
| TDR | 4,672,280 | 4,672,298 | CTGCTGCACCCATGCACTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGGGAAGTGTCTTCACG |
| SD | GGTTCAGTGCTGAGAAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15590A-C4 | VGB6 | C57BL/6NTac | |
| 15590A-G7 | VGB6 | C57BL/6NTac | |
| 15590A-F8 | VGB6 | C57BL/6NTac | |
| 15590A-H8 | VGB6 | C57BL/6NTac | |
| 15590A-A11 | VGB6 | C57BL/6NTac | |
| 15590A-G9 | VGB6 | C57BL/6NTac | |
