15592 Actr2 Status: ES cell colonies screened / QC no positives
| Gene | Actr2 |
| Chromosome | 11 |
| Deletion Start | 20,112,849 |
| Deletion End | 20,100,827 |
| Deletion Size | 12,023 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,112,478 | 20,112,496 | CCTAGCGGCCTTCCTCTTC |
| TUP | 20,112,451 | 20,112,474 | TGTAGCGAAAGATGCCGAATGCGA |
| TUR | 20,112,406 | 20,112,423 | GCCTGACAGCACTTCCTC |
| TDF | 20,100,926 | 20,100,948 | TCTGTTTCCTTCCAGTTTGTGAA |
| TDP | 20,100,875 | 20,100,901 | TCCAGAACACATCTTCCCAGCTTTGGT |
| TDR | 20,100,842 | 20,100,872 | ACTTTGGTGGTTGATCTGATAATAGGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCGGTCTTCCTAGCCG |
| SD | AAGCCTCAAGTCTCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
