| Gene | Canx |
| Chromosome | 11 |
| Deletion Start | 50,311,774 |
| Deletion End | 50,296,228 |
| Deletion Size | 15,547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,310,982 | 50,311,004 | TGACCCATTCCTGCCAATACAAC |
| TUP | 50,310,952 | 50,310,980 | AGGGTCTTCTGTAGCTCTGGATGCCTTGG |
| TUR | 50,310,921 | 50,310,940 | CACAAGCCTGCAATCCCAAG |
| TDF | 50,297,078 | 50,297,099 | ACTGGCAGTCAAGATGAGGAAG |
| TDP | 50,297,050 | 50,297,075 | AGCAAGCCTAAAGCAGAGGTAAGGGA |
| TDR | 50,297,004 | 50,297,026 | GACTGTTTACGCTGTCAGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TTTCATCTCCCAGGGTTCAC |
| SD | AACGGCAATTCATTCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15596A-A12 | VGB6 | C57BL/6NTac | |
| 15596A-H1 | VGB6 | C57BL/6NTac | |
| 15596A-A5 | VGB6 | C57BL/6NTac | |
| 15596A-C9 | VGB6 | C57BL/6NTac | |
| 15596A-B1 | VGB6 | C57BL/6NTac | |
