15597 Aff4 Status: Cancelled
| Gene | Aff4 |
| Chromosome | 11 |
| Deletion Start | 53,397,865 |
| Deletion End | 53,368,667 |
| Deletion Size | 29,199 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,368,916 | 53,368,940 | CAGGCATATTCTCAGGTCTCTTCAG |
| TUP | 53,368,941 | 53,368,966 | AGGACAGAAGGCAATATCAGGTGCCC |
| TUR | 53,369,000 | 53,369,019 | GGTTCCCAGCACCCATGTAG |
| TDF | 53,397,461 | 53,397,483 | AGTAGTGGTACTGAGCATTGAAC |
| TDP | 53,397,484 | 53,397,504 | TAGGCAAACTCTGCTTCTTTA |
| TDR | 53,397,506 | 53,397,524 | GCCAGGACCCTGTTTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TCCTTTCCCTCTGAATGTGC |
| SD | CACGCAAATCTGACAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
