15607 Rabep1 Status: ES cell colonies screened / QC no positives
| Gene | Rabep1 |
| Chromosome | 11 |
| Deletion Start | 70,874,869 |
| Deletion End | 70,844,978 |
| Deletion Size | 29,892 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,845,667 | 70,845,688 | GGCCGAAATCGAGGAGTCAATC |
| TUP | 70,845,692 | 70,845,719 | TTCTCCATTCCGTCTCTACCGTTCAAGC |
| TUR | 70,845,745 | 70,845,766 | AGCTCAGGAGTTTCAGGTCATC |
| TDF | 70,874,118 | 70,874,138 | GCCAAGGGTTGGTGTTTTGAC |
| TDP | 70,874,139 | 70,874,164 | CAAAACAGTCCCTGGCCTAGACCAGA |
| TDR | 70,874,216 | 70,874,240 | CCTGACCACAGTCTGACAATAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTAGGCCAGCGTCACGTGC |
| SD | CAGGGACTGTACCTCAAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
