15620 Aatf Status: ES cell colonies screened / QC no positives
| Gene | Aatf |
| Chromosome | 11 |
| Deletion Start | 84,513,379 |
| Deletion End | 84,509,663 |
| Deletion Size | 3,717 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,512,903 | 84,512,926 | GCCATTTGTCCACATTCTGATGAC |
| TUP | 84,512,865 | 84,512,892 | TTCATAAACCTGTTCTGAGACCGGCTGG |
| TUR | 84,512,829 | 84,512,848 | ACACCTCCCTGACCCAAGTC |
| TDF | 84,509,871 | 84,509,892 | TGCTGCTTAGAAACTGGTGTTC |
| TDP | 84,509,847 | 84,509,869 | TGTCCTTCTTCAGTGCCCAGCCC |
| TDR | 84,509,820 | 84,509,841 | CCCATCTCAGGATCAGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CAATCAACCTTTCTCGCTCC |
| SD | AGTTGATCATTCAGTTTGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
