15620   Aatf     Status: ES cell colonies screened / QC no positives

GeneAatf
Chromosome11
Deletion Start84,326,881
Deletion End84,323,165
Deletion Size3,717
Genome Build37
MGI IDMGI:1929608
NCBI Gene ID56321
BAC ID315K12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF84,326,40584,326,428GCCATTTGTCCACATTCTGATGAC
TUP84,326,36784,326,394TTCATAAACCTGTTCTGAGACCGGCTGG
TUR84,326,33184,326,350ACACCTCCCTGACCCAAGTC
TDF84,323,37384,323,394TGCTGCTTAGAAACTGGTGTTC
TDP84,323,34984,323,371TGTCCTTCTTCAGTGCCCAGCCC
TDR84,323,32284,323,343CCCATCTCAGGATCAGGAAGTC

PCR Primers

PrimerSequence
SUCAATCAACCTTTCTCGCTCC
SDAGTTGATCATTCAGTTTGTTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR98 bp++-
TDFTDR73 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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