| Gene | Gjc1 |
| Chromosome | 11 |
| Deletion Start | 102,801,172 |
| Deletion End | 102,799,890 |
| Deletion Size | 1,283 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,800,824 | 102,800,844 | CAGCTCGGAGCAAACCCTATG |
| TUP | 102,800,798 | 102,800,821 | ATGCGTTGGAAACAGCACCGGGCT |
| TUR | 102,800,772 | 102,800,794 | TCATGGTCCTCTTCCGTTTCTTC |
| TDF | 102,800,433 | 102,800,457 | GCCTCTGCCTATTGCTTAACATTTG |
| TDP | 102,800,399 | 102,800,427 | TGCTTCACTTAGGGTTTGGGACAATTCGA |
| TDR | 102,800,353 | 102,800,374 | AAGCACCCGGATCATCAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CACAGTGTGCTGCATCTGAG |
| SD | AAGTGTAACTACAGCAGTCCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15644A-E8 | VGB6 | C57BL/6NTac | |
| 15644B-A10 | VGB6 | C57BL/6NTac | |
