15647 Nmt1 Status: Cancelled
| Gene | Nmt1 |
| Chromosome | 11 |
| Deletion Start | 103,058,309 |
| Deletion End | 103,028,575 |
| Deletion Size | 29,735 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,028,659 | 103,028,676 | TGCAGCGATTGCGAGAAC |
| TUP | 103,028,678 | 103,028,703 | AGGAGGACAACAGCCACAACAGGAGG |
| TUR | 103,028,736 | 103,028,753 | ACCCAGCTTGTGGGAGAC |
| TDF | 103,058,136 | 103,058,158 | TGCCCTTGTCCTCTTAATGAGTG |
| TDP | 103,058,163 | 103,058,186 | TACCCAGAGTCCTCTGAGGCCAGG |
| TDR | 103,058,245 | 103,058,265 | GCTCAGATGGGAGAACTTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGCAAGCCCTTTACTTGC |
| SD | GGACTATGTAGGACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
