15650 Helz Status: Cancelled
| Gene | Helz |
| Chromosome | 11 |
| Deletion Start | 107,604,359 |
| Deletion End | 107,574,959 |
| Deletion Size | 29,401 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,575,614 | 107,575,633 | TGTGGCGATCTGGAGGAAAG |
| TUP | 107,575,636 | 107,575,658 | AACAGGCAGCCAGTGCAGGGACC |
| TUR | 107,575,685 | 107,575,704 | CCAGCCCAGCTATTGCTCAC |
| TDF | 107,603,668 | 107,603,689 | CTGGATTGTGAGATGGAGAGTC |
| TDP | 107,603,691 | 107,603,720 | AAGAGTGTTTAGCTTTAGCCCTGAACCTGA |
| TDR | 107,603,728 | 107,603,748 | CGAGGACGTTACCCTTGAGAG |
PCR Primers
| Primer | Sequence |
| SU | GTTAGATCTTACACTGTGG |
| SD | TCATTGCCAGTAGAAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
