| Gene | Mycn |
| Chromosome | 12 |
| Deletion Start | 12,940,390 |
| Deletion End | 12,936,946 |
| Deletion Size | 3,445 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,940,337 | 12,940,356 | TCTGCAAGAACCCAGACCTC |
| TUP | 12,940,310 | 12,940,335 | AGTTTGACTCACTGCAGCCCTGCTTC |
| TUR | 12,940,274 | 12,940,292 | GGGACCGCCGAAGTAGAAG |
| TDF | 12,937,576 | 12,937,598 | ACGAGGAGGAAGATGAAGAGGAG |
| TDP | 12,937,542 | 12,937,571 | TCGATGTGGTCACCGTAGAGAAGAGACGTT |
| TDR | 12,937,514 | 12,937,535 | GTGGTTACCGCCTTGTTGTTAG |
PCR Primers
| Primer | Sequence |
| SU | CTGGGCTAGAGAGCAAGGAC |
| SD | GACCGGAACAAGGTGACTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15661A-D3 | VGB6 | C57BL/6NTac | |
| 15661A-D11 | VGB6 | C57BL/6NTac | |
| 15661A-H12 | VGB6 | C57BL/6NTac | |
| 15661A-E3 | VGB6 | C57BL/6NTac | |
